Blar i forfatter "Lahrberg, Marcel"

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    • Accurate estimation of the illumination pattern’s orientation and wavelength in sinusoidal structured illumination microscopy 

      Lahrberg, Marcel; Singh, Mandeep; Khare, Kedar; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-01-05)
      Structured illumination microscopy is able to improve the spatial resolution of wide-field fluorescence imaging by applying sinusoidal stripe pattern illumination to the sample. The corresponding computational image reconstruction requires precise knowledge of the pattern’s parameters, which are its phase (φ) and wave vector (<b>p</b>). Here, a computationally inexpensive method for estimation of ...

    • Improving the space-bandwidth product of structured illumination microscopy using a transillumination configuration 

      Joseph, Joby; Faiz, Kandankulangara P.; Lahrberg, Marcel; Tinguely, Jean-Claude; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-18)
      Applying structured instead of plane illumination in widefield optical fluorescence microscopy can improve the spatial resolution beyond what is known as the Abbe limit. In general it is not only the resolution of an imaging system that is of interest but also its field of view (FOV). These two parameters are expressed in the space-bandwidth product (SBP). Here we introduce a modified structured ...

    • Novel strategies for super-resolution fluorescence microscopy 

      Lahrberg, Marcel (Doctoral thesis; Doktorgradsavhandling, 2019-12-11)
      <p>Minimally invasive technologies to characterize the structure and functionality of biological samples on the cellular and molecular scale are fundamental to life sciences. Optical fluorescence imaging at visible wavelengths is able to acquire to do so. Due to the wavelike nature of light the maximum achievable resolution in conventional microscopy is limited by diffraction, about half the wavelength ...

    • Photonic-chip based free space beam shaping and steering for advanced optical microscopy application 

      Lahrberg, Marcel; Dullo, Firehun Tsige; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-12)
      Photonic-chip-based light illumination has recently found applications in optical microscopy and nanoscopy methodologies. The photonic chip removes the dependency on imaging objective lenses to generate the required illumination patterns for different microscopy methods. Until now, all the reported chip-based optical microscopy methods exploit the evanescent field present on top of a waveguide surface ...

    • Structured illumination microscopy using a photonic chip 

      Helle, Øystein Ivar; Dullo, Firehun Tsige; Lahrberg, Marcel; Tinguely, Jean-Claude; Hellesø, Olav Gaute; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-11)
      Structured illumination microscopy (SIM) enables live-cell super-resolution imaging of subcellular structures at high speeds. At present, linear SIM uses free-space optics to illuminate the sample with the desired light patterns; however, such arrangements are prone to misalignment and add cost and complexity to the microscope. Here, we present an alternative photonic chip-based two-dimensional SIM ...